Application
ABHD4 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ABHD4 antibody detected the ABHD4 protein (arrow).Application
ABHD4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ABHD4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ABHD4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABHD4; Abhydrolase domain-containing protein 4; Alpha/beta-hydrolase 4; Lyso-N-acylphosphatidylethanolamine lipase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ABHD4 Abhydrolase domain-containing protein 4 Alpha/beta-hydrolase 4 Lyso-N-acylphosphatidylethanolamine lipase |
|---|---|
| Molecular Weight(MW) | 38794 Da |
| Function | Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity) |
| Tissue Specificity | This ABHD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-127 amino acids from the Central region of human ABHD4. |
| Entrez Gene | 63874 |
|---|---|
| Nucleotide Accession | NP_071343.2 |
Application
ABHD4 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ABHD4 antibody detected the ABHD4 protein (arrow).
Application
ABHD4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ABHD4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
