Application
Confocal immunofluorescent analysis of ACAT2 Antibody (Center)(Cat#AP6530c) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of ACAT2 Antibody (Center) in A2058,HepG2 cell line lysates(35ug/lane). ACAT2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with ACAT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ACAT2 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACAT2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACAT2; ACTL; Acetyl-CoA acetyltransferase, cytosolic; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHCFC |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTL |
|---|---|
| Alternative Names | ACAT2 ACTL Acetyl-CoA acetyltransferase cytosolic Acetyl-CoA transferase-like protein Cytosolic acetoacetyl-CoA thiolase |
| Molecular Weight(MW) | 41351 Da |
| Tissue Specificity | This ACAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202~232 amino acids from the Center region of human ACAT2. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 39 |
|---|
Application
Confocal immunofluorescent analysis of ACAT2 Antibody (Center)(Cat#AP6530c) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of ACAT2 Antibody (Center) in A2058,HepG2 cell line lysates(35ug/lane). ACAT2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with ACAT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ACAT2 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
