Application
Western blot analysis of ACDC antibody (N-term) (Cat.# AP6531a) in NCI-H460 cell line lysates (35ug/lane). ACDC (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with ACDC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ACDC Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACDC Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; Gelatin-bindi |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACDC ACRP30 APM1 GBP28 |
|---|---|
| Alternative Names | ADIPOQ ACDC ACRP30 APM1 GBP28 Adiponectin 30 kDa adipocyte complement-related protein Adipocyte complement-related 30 kDa protein Adipocyte C1q and collagen domain-containing protein Adipose most abundant gene transcript 1 protein Gelatin-bindi |
| Molecular Weight(MW) | 26414 Da |
| Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW |
| Tissue Specificity | Synthesized exclusively by adipocytes and secreted into plasma |
| Cellular Localization | Secreted. |
| Entrez Gene | 9370 |
|---|
Application
Western blot analysis of ACDC antibody (N-term) (Cat.# AP6531a) in NCI-H460 cell line lysates (35ug/lane). ACDC (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with ACDC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ACDC Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
