Application
Western blot analysis of ACLY Antibody (N-term) in Hela cell line lysates (35ug/lane). ACLY (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of hela cells using ACLY Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACLY Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACLY; ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; Citrate cleavage enzyme |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACLY ATP-citrate synthase ATP-citrate (pro-S-)-lyase Citrate cleavage enzyme |
|---|---|
| Molecular Weight(MW) | 120839 Da |
| Function | ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine |
| Tissue Specificity | This ACLY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147~177 amino acids from the N-terminal region of human ACLY. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 47 |
|---|
Application
Western blot analysis of ACLY Antibody (N-term) in Hela cell line lysates (35ug/lane). ACLY (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of hela cells using ACLY Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
