Application
ACOX1 Monoclonal Antibody(Cat. #AM1847b) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the ACOX1 antibody detected the ACOX1 protein (arrow).(1ug/ml)1:390Application
ACOX1 Monoclonal Antibody (Cat. #AM1847b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACOX1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ACOX1 Antibody (Cat#AM1847b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ACOX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACOX1; ACOX; Peroxisomal acyl-coenzyme A oxidase 1; Palmitoyl-CoA oxidase; Straight-chain acyl-CoA oxidase |
| Clonality | Monoclonal |
|---|---|
| Isotype | Ig |
| Clone Number | 153CT43.1.1 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACOX |
|---|---|
| Alternative Names | ACOX1 ACOX Peroxisomal acyl-coenzyme A oxidase 1 Palmitoyl-CoA oxidase Straight-chain acyl-CoA oxidase |
| Molecular Weight(MW) | 74424 Da |
| Function | Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA |
| Tissue Specificity | Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart |
| Cellular Localization | Peroxisome. |
| Entrez Gene | 51 |
|---|---|
| Nucleotide Accession | NP_009223.2 |
Application
ACOX1 Monoclonal Antibody(Cat. #AM1847b) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the ACOX1 antibody detected the ACOX1 protein (arrow).(1ug/ml)1:390
Application
ACOX1 Monoclonal Antibody (Cat. #AM1847b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACOX1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ACOX1 Antibody (Cat#AM1847b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
