Application
Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (RB04651). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (RB04652). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies).| Product Name | ACOX1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACOX1; ACOX; Peroxisomal acyl-coenzyme A oxidase 1; Palmitoyl-CoA oxidase; Straight-chain acyl-CoA oxidase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACOX |
|---|---|
| Alternative Names | ACOX1 ACOX Peroxisomal acyl-coenzyme A oxidase 1 Palmitoyl-CoA oxidase Straight-chain acyl-CoA oxidase |
| Molecular Weight(MW) | 74424 Da |
| Function | Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA |
| Tissue Specificity | Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart |
| Cellular Localization | Peroxisome. |
| Entrez Gene | 51 |
|---|
Application
Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (RB04651). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (RB04652). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
