Application
FACL4-E251 western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FACL4 antibody detected the FACL4 protein (arrow).Application
Confocal immunofluorescent analysis of ACSL4 (FACL4) Antibody (Center) (Cat#AP2536b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ACSL4 (FACL4) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACSL4; ACS4; FACL4; LACS4; Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACS4 FACL4 LACS4 |
|---|---|
| Alternative Names | ACSL4 ACS4 FACL4 LACS4 Long-chain-fatty-acid--CoA ligase 4 Long-chain acyl-CoA synthetase 4 |
| Molecular Weight(MW) | 79188 Da |
| Function | Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation Preferentially uses arachidonate and eicosapentaenoate as substrates |
| Tissue Specificity | This ACSL4 (FACL4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244~274 amino acids from the center region of human FACL4. |
| Cellular Localization | Mitochondrion outer membrane; Single-pass type III membrane protein (By similarity). Peroxisome membrane; Single-pass type III membrane protein (By similarity). Microsome membrane; Single-pass type III membrane protein (By similarity) Endoplasmic reticulum membrane; Single-pass type III membrane protein (By similarity) |
| Entrez Gene | 2182 |
|---|
Application
FACL4-E251 western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FACL4 antibody detected the FACL4 protein (arrow).
Application
Confocal immunofluorescent analysis of ACSL4 (FACL4) Antibody (Center) (Cat#AP2536b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
