WB
Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACSL4/FACL4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. ACSL4/FACL4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody . ACSL4/FACL4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. ACSL4/FACL4 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. ACSL4/FACL4 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to ACSL4/FACL4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody ( 1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.FC
Flow Cytometry analysis of U87 cells using anti-ACSL4/FACL4 antibody. Overlay histogram showing U87 cells stained with Rabbit polyclonal antibody to ACSL4/FACL4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to ACSL4/FACL4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FACL4/ACSL4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACSL4 |
|---|---|
| Gene Synonyms | ACS4 FACL4 LACS4 MRX63 MRX68 XLID63 |
| Gene Full Name | acyl-CoA synthetase long chain family member 4 |
| Gene Summary | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016] |
| Alternative Names | ACS4 ACSL4 ACSL4/FACL4 FACL4 LACS 4 LACS4 MRX63 MRX68 |
| Molecular Weight(MW) | 79 kDa |
| Cellular Localization | Cell membrane,Endoplasmic reticulum,Membrane,Mitochondrion,Mitochondrion outer membrane |
WB
Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACSL4/FACL4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. ACSL4/FACL4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody . ACSL4/FACL4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. ACSL4/FACL4 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody. ACSL4/FACL4 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ACSL4/FACL4 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to ACSL4/FACL4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody ( 1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
FC
Flow Cytometry analysis of U87 cells using anti-ACSL4/FACL4 antibody. Overlay histogram showing U87 cells stained with Rabbit polyclonal antibody to ACSL4/FACL4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
