Application
Western blot analysis of anti-FACL5 Antibody (N-term) in K562 cell line lysates (35ug/lane). FACL5 (arrow) was detected using the purified Pab.Application
Western blot analysis of anti-FACL5 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). FACL5 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | ACSL5 (FACL5) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACSL5; ACS5; FACL5; Long-chain-fatty-acid--CoA ligase 5; Long-chain acyl-CoA synthetase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~200 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACS5 FACL5 |
|---|---|
| Alternative Names | ACSL5 ACS5 FACL5 Long-chain-fatty-acid--CoA ligase 5 Long-chain acyl-CoA synthetase 5 |
| Molecular Weight(MW) | 75991 Da |
| Function | Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells |
| Tissue Specificity | This ACSL5 (FACL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46~75 amino acids from the N-terminal region of of human FACL5. |
| Cellular Localization | Mitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane; Single-pass type III membrane protein (By similarity). Endoplasmic reticulum membrane; Single- pass type III membrane protein (By similarity) |
| Entrez Gene | 51703 |
|---|
Application
Western blot analysis of anti-FACL5 Antibody (N-term) in K562 cell line lysates (35ug/lane). FACL5 (arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-FACL5 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). FACL5 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:50~200 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
