Application
Western blot analysis of ACTG1 antibody (N-term) (Cat.# AP6532a) in HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ACTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ACTG1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.Application
Confocal immunofluorescent analysis of ACTG1 Antibody (N-term)(Cat#AP6532a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ACTG1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin; Actin, cytoplasmic 2, N-terminally processed |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTB ACTG |
|---|---|
| Alternative Names | ACTG1 ACTB ACTG Actin cytoplasmic 2 Gamma-actin Actin cytoplasmic 2 N-terminally processed |
| Molecular Weight(MW) | 41793 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41~71 amino acids from the N-terminal region of human ACTG1. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 71 |
|---|
Application
Western blot analysis of ACTG1 antibody (N-term) (Cat.# AP6532a) in HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ACTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ACTG1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.
Application
Confocal immunofluorescent analysis of ACTG1 Antibody (N-term)(Cat#AP6532a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
