Application
ACTB Antibody western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ACTB antibody detected the ACTB protein (arrow).Application
ACTB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of ACTB (arrow) using rabbit polyclonal ACTB Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTB gene.Application
ACTB Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACTB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTB; Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACTB Actin cytoplasmic 1 Beta-actin Actin cytoplasmic 1 N-terminally processed |
|---|---|
| Molecular Weight(MW) | 41737 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB. |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 60 |
|---|---|
| Nucleotide Accession | NP_001092.1 |
Application
ACTB Antibody western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ACTB antibody detected the ACTB protein (arrow).
Application
ACTB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of ACTB (arrow) using rabbit polyclonal ACTB Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTB gene.
Application
ACTB Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
