Application
Western blot analysis of anti-ACTN4 Antibody (N-term) in Hela cell line lysates (35ug/lane). ACTN4 (arrow) was detected using the purified Pab.| Product Name | ACTN4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTN4; Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACTN4 Alpha-actinin-4 F-actin cross-linking protein Non-muscle alpha-actinin 4 |
|---|---|
| Molecular Weight(MW) | 104854 Da |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC- 2, and bladder cancer KU-7) |
| Entrez Gene | 81 |
|---|
Application
Western blot analysis of anti-ACTN4 Antibody (N-term) in Hela cell line lysates (35ug/lane). ACTN4 (arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
