Application
Western blot analysis of ACTR3 Antibody (C-term) in A2058 cell line lysates (35ug/lane). ACTR3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of hela cells using ACTR3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACTR3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTR3; ARP3; Actin-related protein 3; Actin-like protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARP3 |
|---|---|
| Alternative Names | ACTR3 ARP3 Actin-related protein 3 Actin-like protein 3 |
| Molecular Weight(MW) | 47371 Da |
| Function | Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis |
| Tissue Specificity | This ACTR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387~416 amino acids from the C-terminal region of human ACTR3. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell projection. |
| Entrez Gene | 10096 |
|---|
Application
Western blot analysis of ACTR3 Antibody (C-term) in A2058 cell line lysates (35ug/lane). ACTR3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of hela cells using ACTR3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
