Application
ADH1C Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ADH1C antibody detected the ADH1C protein (arrow).Application
Western blot analysis of ADH1C (arrow) using rabbit polyclonal ADH1C Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.Application
ADH1C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH1C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ADH1C Antibody (Center)(Cat#AP5534c) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
ADH1C Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ADH1C Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH1C; ADH3; Alcohol dehydrogenase 1C; Alcohol dehydrogenase subunit gamma |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADH3 |
|---|---|
| Alternative Names | ADH1C ADH3 Alcohol dehydrogenase 1C Alcohol dehydrogenase subunit gamma |
| Molecular Weight(MW) | 39868 Da |
| Tissue Specificity | This ADH1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-268 amino acids from the Central region of human ADH1C. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 126 |
|---|---|
| Nucleotide Accession | NP_000660.1 |
Application
ADH1C Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ADH1C antibody detected the ADH1C protein (arrow).
Application
Western blot analysis of ADH1C (arrow) using rabbit polyclonal ADH1C Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.
Application
ADH1C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH1C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ADH1C Antibody (Center)(Cat#AP5534c) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
ADH1C Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
