Application
Western blot analysis of ADH5 Antibody (Center) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab.Application
Western blot analysis of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using ADH5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ADH5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH5; ADHX; FDH; Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; S-(hydroxymethyl)glutathione dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADHX FDH |
|---|---|
| Alternative Names | ADH5 ADHX FDH Alcohol dehydrogenase class-3 Alcohol dehydrogenase 5 Alcohol dehydrogenase class chi chain Alcohol dehydrogenase class-III Glutathione-dependent formaldehyde dehydrogenase S-(hydroxymethyl)glutathione dehydrogenase |
| Molecular Weight(MW) | 39724 Da |
| Function | Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione |
| Tissue Specificity | This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219~248 amino acids from the Center region of human ADH5. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 128 |
|---|
Application
Western blot analysis of ADH5 Antibody (Center) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab.
Application
Western blot analysis of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using ADH5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
