Application
ADH7 Antibody (C-Term) western blot analysis in Jurkat,CEM,NCI-H460,K562,MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ADH7 antibody detected the ADH7 protein (arrow).Application
ADH7 Antibody (C-Term) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH7 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ADH7 Antibody (C-Term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of ADH7 Antibody (C-Term) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ADH7 Antibody (C-Term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH7; Alcohol dehydrogenase class 4 mu/sigma chain; Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Retinol dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ADH7 Alcohol dehydrogenase class 4 mu/sigma chain Alcohol dehydrogenase class IV mu/sigma chain Gastric alcohol dehydrogenase Retinol dehydrogenase |
|---|---|
| Molecular Weight(MW) | 41481 Da |
| Function | Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism |
| Tissue Specificity | Preferentially expressed in stomach. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 131 |
|---|
Application
ADH7 Antibody (C-Term) western blot analysis in Jurkat,CEM,NCI-H460,K562,MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ADH7 antibody detected the ADH7 protein (arrow).
Application
ADH7 Antibody (C-Term) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH7 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ADH7 Antibody (C-Term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of ADH7 Antibody (C-Term) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
