Application
Western blot analysis of ADHFE1 Antibody (Center) in mouse heart tissue lysates (35ug/lane). ADHFE1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADHFE1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ADHFE1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADHFE1; Hydroxyacid-oxoacid transhydrogenase, mitochondrial; Alcohol dehydrogenase iron-containing protein 1; Fe-containing alcohol dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ADHFE1 Hydroxyacid-oxoacid transhydrogenase mitochondrial Alcohol dehydrogenase iron-containing protein 1 Fe-containing alcohol dehydrogenase |
|---|---|
| Molecular Weight(MW) | 50308 Da |
| Function | Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2- hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3- hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities |
| Tissue Specificity | Only expressed in adult liver. |
| Cellular Localization | Mitochondrion (By similarity). |
| Entrez Gene | 137872 |
|---|
Application
Western blot analysis of ADHFE1 Antibody (Center) in mouse heart tissue lysates (35ug/lane). ADHFE1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADHFE1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
