Application
Western blot analysis of ADRA1B Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ADRA1B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ADRA1B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADRA1B; Alpha-1B adrenergic receptor; Alpha-1B adrenoreceptor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ADRA1B Alpha-1B adrenergic receptor Alpha-1B adrenoreceptor |
|---|---|
| Molecular Weight(MW) | 56836 Da |
| Function | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes |
| Tissue Specificity | This ADRA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276~306 amino acids from the Central region of human ADRA1B. |
| Cellular Localization | Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes |
| Entrez Gene | 147 |
|---|
Application
Western blot analysis of ADRA1B Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ADRA1B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
