Application
Western blot analysis of ADRA2B Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). ADRA2B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-231 cells using ADRA2B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of ADRA2B Antibody (Center)(Cat#AP8566c) with MDA-MB231 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ADRA2B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADRA2B; ADRA2L1; ADRA2RL1; Alpha-2B adrenergic receptor; Alpha-2 adrenergic receptor subtype C2; Alpha-2B adrenoreceptor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADRA2L1 ADRA2RL1 |
|---|---|
| Alternative Names | ADRA2B ADRA2L1 ADRA2RL1 Alpha-2B adrenergic receptor Alpha-2 adrenergic receptor subtype C2 Alpha-2B adrenoreceptor |
| Molecular Weight(MW) | 49954 Da |
| Function | Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol |
| Tissue Specificity | This ADRA2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349~378 amino acids from the Center region of human ADRA2B. |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Note=Interaction with RAB26 mediates transport from the Golgi to the cell membrane |
| Entrez Gene | 151 |
|---|
Application
Western blot analysis of ADRA2B Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). ADRA2B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-231 cells using ADRA2B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of ADRA2B Antibody (Center)(Cat#AP8566c) with MDA-MB231 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
