Application
Western blot analysis of AF5q31 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). AF5q31 (arrow) was detected using the purified Pab.| Product Name | AF5q31 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AFF4; AF5Q31; MCEF; AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Major CDK9 elongation factor-associated protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AF5Q31 MCEF |
|---|---|
| Alternative Names | AFF4 AF5Q31 MCEF AF4/FMR2 family member 4 ALL1-fused gene from chromosome 5q31 protein Major CDK9 elongation factor-associated protein |
| Molecular Weight(MW) | 127459 Da |
| Function | Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression |
| Tissue Specificity | Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain |
| Cellular Localization | Nucleus. |
| Entrez Gene | 27125 |
|---|
Application
Western blot analysis of AF5q31 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). AF5q31 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
