Application
Western blot analysis of AFMID Antibody (N-term) in mouse liver tissue lysates (35ug/lane). AFMID (arrow) was detected using the purified Pab.Application
AFMID Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AFMID Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AFMID; Probable arylformamidase; Kynurenine formamidase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AFMID Probable arylformamidase Kynurenine formamidase |
|---|---|
| Molecular Weight(MW) | 33992 Da |
| Function | Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites (By similarity) |
| Tissue Specificity | This AFMID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~92 amino acids from the N-terminal region of human AFMID. |
| Cellular Localization | Cytoplasm, cytosol (By similarity). Nucleus (By similarity). Note=Predominantly cytosolic. Some fraction is nuclear (By similarity) |
| Entrez Gene | 125061 |
|---|
Application
Western blot analysis of AFMID Antibody (N-term) in mouse liver tissue lysates (35ug/lane). AFMID (arrow) was detected using the purified Pab.
Application
AFMID Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
