Application
AGBL5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AGBL5 antibody detected the AGBL5 protein (arrow).Application
AGBL5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AGBL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of AGBL5 Antibody (N-term)(Cat#AP11785a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | AGBL5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CCP5 |
|---|---|
| Alternative Names | AGBL5 CCP5 Cytosolic carboxypeptidase-like protein 5 ATP/GTP-binding protein-like 5 |
| Molecular Weight(MW) | 97534 Da |
| Function | Metallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post- translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6 (By similarity) |
| Tissue Specificity | Expressed in brain. |
| Cellular Localization | Cytoplasm, cytosol (By similarity). Nucleus (By similarity). Note=Mainly cytoplasmic. Slight accumulation in the nucleus is observed (By similarity) |
| Entrez Gene | 60509 |
|---|---|
| Nucleotide Accession | NP_068603.4 |
Application
AGBL5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AGBL5 antibody detected the AGBL5 protein (arrow).
Application
AGBL5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AGBL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of AGBL5 Antibody (N-term)(Cat#AP11785a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
