Application
Western blot analysis of AGO1 Antibody (N-term) in K562 cell line lysates (35ug/lane). AGO1 (arrow) was detected using the purified Pab.Application
AGO1 Antibody (N-term) (RB18965) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | AGO1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AGO1 |
|---|---|
| Alternative Names | EIF2C1 AGO1 Protein argonaute-1 Eukaryotic translation initiation factor 2C 1 Putative RNA-binding protein Q99 |
| Molecular Weight(MW) | 97214 Da |
| Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs) |
| Tissue Specificity | This AGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34~64 amino acids from the N-terminal region of human AGO1. |
| Cellular Localization | Cytoplasm, P-body. |
| Entrez Gene | 26523 |
|---|
Application
Western blot analysis of AGO1 Antibody (N-term) in K562 cell line lysates (35ug/lane). AGO1 (arrow) was detected using the purified Pab.
Application
AGO1 Antibody (N-term) (RB18965) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
