Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-AKR1A1 Antibody (C-term) in Y79 cell line lysates (35ug/lane). AKR1A1(arrow) was detected using the purified Pab (1:60dilution).Application
Western blot analysis of AKR1A1 (arrow) using rabbit polyclonal AKR1A1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1A1 gene.| Product Name | AKR1A1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALDR1, ALR |
|---|---|
| Alternative Names | AKR1A1 ALDR1 ALR Alcohol dehydrogenase [NADP(+)] Aldehyde reductase Aldo-keto reductase family 1 member A1 |
| Molecular Weight(MW) | 36573 Da |
| Function | Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4- nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D- glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN) |
| Tissue Specificity | Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-AKR1A1 Antibody (C-term) in Y79 cell line lysates (35ug/lane). AKR1A1(arrow) was detected using the purified Pab (1:60dilution).
Application
Western blot analysis of AKR1A1 (arrow) using rabbit polyclonal AKR1A1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1A1 gene.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
