Application
AKR1C3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKR1C3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
AKR1C3 Antibody (Center) western blot analysis in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates the AKR1C3 antibody detected the AKR1C3 protein (arrow).| Product Name | AKR1C3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DDH1, HSD17B5, KIAA0119, PGFS |
|---|---|
| Alternative Names | AKR1C3 DDH1 HSD17B5 KIAA0119 PGFS Aldo-keto reductase family 1 member C3 17-beta-hydroxysteroid dehydrogenase type 5 3-alpha-HSD type II brain 3-alpha-hydroxysteroid dehydrogenase type 2 Chlordecone reductase homolog HAKRb Dihydrodiol dehydroge |
| Molecular Weight(MW) | 36853 Da |
| Function | Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone |
| Tissue Specificity | Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 8644 |
|---|---|
| Nucleotide Accession | NP_003730.4 |
Application
AKR1C3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKR1C3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
AKR1C3 Antibody (Center) western blot analysis in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates the AKR1C3 antibody detected the AKR1C3 protein (arrow).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
