Application
Western blot analysis of ALDH1A1 (arrow) using rabbit polyclonal ALDH1A1 Antibody (Center) (Cat# AP1465c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Immunofluorescence analysis of anti-ALDH1A1 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Application
Flow cytometric analysis of HepG2 cells using ALDH1A1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ALDH1A1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB,IHC,IF,FC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALDC, ALDH1, PUMB1 |
|---|---|
| Alternative Names | ALDH1A1 ALDC ALDH1 PUMB1 Retinal dehydrogenase 1 ALDH-E1 ALHDII Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase cytosolic |
| Molecular Weight(MW) | 54862 Da |
| Function | Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity) |
| Tissue Specificity | This ALDH1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309~339 amino acids from the center region of human ALDH1A1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 216 |
|---|
Application
Western blot analysis of ALDH1A1 (arrow) using rabbit polyclonal ALDH1A1 Antibody (Center) (Cat# AP1465c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Immunofluorescence analysis of anti-ALDH1A1 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Application
Flow cytometric analysis of HepG2 cells using ALDH1A1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
