Application
Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1846b ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.Application
ALDH1A1 Monoclonal Antibody(Cat. #AM1846b) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow).Application
ALDH1A1 Monoclonal Antibody (Cat. #AM1846b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ALDH1A1 Antibody (Cat#AM1846b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ALDH1A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 152CT1.2.2 |
| Host Species | Mouse |
| Tested Applications | IFWBIHC |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALDC, ALDH1, PUMB1 |
|---|---|
| Alternative Names | ALDH1A1 ALDC ALDH1 PUMB1 Retinal dehydrogenase 1 ALDH-E1 ALHDII Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase cytosolic |
| Molecular Weight(MW) | 54862 Da |
| Function | Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity) |
| Tissue Specificity | This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 216 |
|---|---|
| Nucleotide Accession | NP_000680.2 |
Application
Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1846b ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
Application
ALDH1A1 Monoclonal Antibody(Cat. #AM1846b) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow).
Application
ALDH1A1 Monoclonal Antibody (Cat. #AM1846b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ALDH1A1 Antibody (Cat#AM1846b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
