Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-ALDH4A1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ALDH4A1(arrow) was detected using the purified Pab.Application
Western blot analysis of ALDH4A1 (arrow) using rabbit polyclonal ALDH4A1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALDH4A1 gene.| Product Name | ALDH4A1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALDH4, P5CDH |
|---|---|
| Alternative Names | ALDH4A1 ALDH4 P5CDH Delta-1-pyrroline-5-carboxylate dehydrogenase mitochondrial Aldehyde dehydrogenase family 4 member A1 |
| Molecular Weight(MW) | 61719 Da |
| Function | Irreversible conversion of delta-1-pyrroline-5- carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes |
| Tissue Specificity | Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 8659 |
|---|
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-ALDH4A1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ALDH4A1(arrow) was detected using the purified Pab.
Application
Western blot analysis of ALDH4A1 (arrow) using rabbit polyclonal ALDH4A1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALDH4A1 gene.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
