Application
Western blot analysis of ALK(Center)(arrow) using rabbit polyclonal ALK(Center) Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALK (Center) gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with ALK antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ALK Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALK; ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; CD_antigen=CD246; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ALK ALK tyrosine kinase receptor Anaplastic lymphoma kinase CD_antigen=CD246 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 176442 Da |
| Function | Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK |
| Tissue Specificity | Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway |
| Entrez Gene | 238 |
|---|
Application
Western blot analysis of ALK(Center)(arrow) using rabbit polyclonal ALK(Center) Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALK (Center) gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with ALK antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
