Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Alpha-Tubulin (p272) RabMAb (Cat. #AJ1034b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Product Name | Alpha-Tubulin Antibody Phospho (pY272) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUBA1B, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1 |
| Modification | p-Y272 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1334(2)Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TUBA1B Tubulin alpha-1B chain Tubulin alpha-ubiquitous chain Alpha-tubulin ubiquitous Tubulin K-alpha-1 |
|---|---|
| Molecular Weight(MW) | 50152 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain |
| Tissue Specificity | A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha-tubulin was used as an immunogen. This antibody detects alpha-tubulin phosphorylated at tyrosine 272. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 10376 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Alpha-Tubulin (p272) RabMAb (Cat. #AJ1034b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Application Notes | WB:1:2500~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
