Application
A. Western blot analysis on HeLa cell lysate using anti-Alpha-1 Catenin RabMAb (Cat. #AJ1026a), dilution 1:50,000.Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Alpha-1 Catenin RabMAb (Cat. #AJ1026a).| Product Name | Alpha-1 Catenin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTNNA1, Catenin alpha-1, Cadherin-associated protein;Alpha E-catenin;NY-REN-13 antigen |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1793Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CTNNA1 Catenin alpha-1 Cadherin-associated protein Alpha E-catenin NY-REN-13 antigen |
|---|---|
| Molecular Weight(MW) | 100071 Da |
| Function | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments May play a crucial role in cell differentiation |
| Tissue Specificity | Expressed ubiquitously in normal tissues. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries |
| Entrez Gene | 1495 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Alpha-1 Catenin RabMAb (Cat. #AJ1026a), dilution 1:50,000.
Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Alpha-1 Catenin RabMAb (Cat. #AJ1026a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
