Application
Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene.Application
ALPL Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ALPL antibody detected the ALPL protein (arrow).Application
Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat#AP1474c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
ALPL Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ALPL Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALPL; Alkaline phosphatase, tissue-nonspecific isozyme; Alkaline phosphatase liver/bone/kidney isozyme |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ALPL Alkaline phosphatase tissue-nonspecific isozyme Alkaline phosphatase liver/bone/kidney isozyme |
|---|---|
| Molecular Weight(MW) | 57305 Da |
| Function | This isozyme may play a role in skeletal mineralization. |
| Tissue Specificity | This ALPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~254 amino acids from the Center region of human ALPL. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Entrez Gene | 249 |
|---|
Application
Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene.
Application
ALPL Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ALPL antibody detected the ALPL protein (arrow).
Application
Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat#AP1474c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
ALPL Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
