Application
A. Western blot analysis on (A) NIH 3T3 (B) HeLa (C) C6 lysates using anti-alpha-actinin 1 RabMAb (Cat. #AJ1029a), dilution 1:10,000.| Product Name | Alpha-Actinin-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTN1, Alpha-actinin-1, Alpha-actinin cytoskeletal isoform;Non-muscle alpha-actinin-1;F-actin cross-linking protein |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2527Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACTN1 Alpha-actinin-1 Alpha-actinin cytoskeletal isoform Non-muscle alpha-actinin-1 F-actin cross-linking protein |
|---|---|
| Molecular Weight(MW) | 103058 Da |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein |
| Tissue Specificity | A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 1 was used as an immunogen. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Cell membrane (By similarity). Cell projection, ruffle (By similarity). Note=Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures (By similarity) |
| Entrez Gene | 87 |
|---|
Application
A. Western blot analysis on (A) NIH 3T3 (B) HeLa (C) C6 lysates using anti-alpha-actinin 1 RabMAb (Cat. #AJ1029a), dilution 1:10,000.| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
