Application
Fluorescent confocal image of HeLa cells stained with ALPP (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2022a ALPP (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALPP immunoreactivity is localized to the cytoplasm of HeLa cells.Application
ALPP Antibody (N-term) (Cat. #AM2022a) western blot analysis in mouse placenta tissue lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).| Product Name | ALPP Antibody (N-term)(Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALPP; PLAP; Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 421CT8.1.1 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PLAP |
|---|---|
| Alternative Names | ALPP PLAP Alkaline phosphatase placental type Alkaline phosphatase Regan isozyme Placental alkaline phosphatase 1 |
| Molecular Weight(MW) | 57954 Da |
| Tissue Specificity | This ALPP Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the N-term region of human ALPP. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Entrez Gene | 250 |
|---|---|
| Nucleotide Accession | NP_001623.3 |
Application
Fluorescent confocal image of HeLa cells stained with ALPP (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2022a ALPP (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALPP immunoreactivity is localized to the cytoplasm of HeLa cells.
Application
ALPP Antibody (N-term) (Cat. #AM2022a) western blot analysis in mouse placenta tissue lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
