Application
PRKAB2 Antibody (A18) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the PRKAB2 antibody detected the PRKAB2 protein (arrow).Application
PRKAB2 Antibody (A18) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PRKAB2 antibody detected the PRKAB2 protein (arrow).| Product Name | AMPK beta2 (PRKAB2) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAB2; 5'-AMP-activated protein kinase subunit beta-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRKAB2 5'-AMP-activated protein kinase subunit beta-2 |
|---|---|
| Molecular Weight(MW) | 30302 Da |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) |
| Tissue Specificity | This PRKAB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human PRKAB2. |
Application
PRKAB2 Antibody (A18) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the PRKAB2 antibody detected the PRKAB2 protein (arrow).
Application
PRKAB2 Antibody (A18) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PRKAB2 antibody detected the PRKAB2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
