Application
A. Western blot analysis of anti- AMPK gamma-1 (N-term) RabMAb (Cat. #AJ1039b), dilution 1:2,000. A: Jurkat B: HelaApplication
B. Immunohistochemical analysis of paraffin-embedded human skeletal muscles using anti-AMPK gamma-1 (N-term) RabMAb (Cat. #AJ1039b).| Product Name | AMPK gamma-1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAG1, 5'-AMP-activated protein kinase subunit gamma-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y307 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRKAG1 5'-AMP-activated protein kinase subunit gamma-1 |
|---|---|
| Molecular Weight(MW) | 37579 Da |
| Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive |
| Tissue Specificity | A synthetic peptide corresponding to residues in the N-term of human AMPK gamma-1 subunits was used as an immunogen |
| Entrez Gene | 5571 |
|---|
Application
A. Western blot analysis of anti- AMPK gamma-1 (N-term) RabMAb (Cat. #AJ1039b), dilution 1:2,000. A: Jurkat B: Hela
Application
B. Immunohistochemical analysis of paraffin-embedded human skeletal muscles using anti-AMPK gamma-1 (N-term) RabMAb (Cat. #AJ1039b).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
