Application
ANAPC5 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANAPC5 antibody detected the ANAPC5 protein (arrow).Application
ANAPC5 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ANAPC5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANAPC5; APC5; Anaphase-promoting complex subunit 5; Cyclosome subunit 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APC5 |
|---|---|
| Alternative Names | ANAPC5 APC5 Anaphase-promoting complex subunit 5 Cyclosome subunit 5 |
| Molecular Weight(MW) | 85077 Da |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains |
| Tissue Specificity | This ANAPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human ANAPC5. |
| Entrez Gene | 51433 |
|---|---|
| Nucleotide Accession | NP_057321.2 |
Application
ANAPC5 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANAPC5 antibody detected the ANAPC5 protein (arrow).
Application
ANAPC5 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
