Application
Western blot analysis of AGR2 Antibody (N-term) in T47D cell line lysates (35ug/lane). AGR2 (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of AGR2(arrow) using rabbit polyclonal AGR2 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AGR2 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Anterior Gradient 2 (AGR2) Antibody (N-term)(Cat#AP6279a) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Anterior Gradient 2 (AGR2) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGR2; AG2; Anterior gradient protein 2 homolog; HPC8; Secreted cement gland protein XAG-2 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AG2 |
|---|---|
| Alternative Names | AGR2 AG2 Anterior gradient protein 2 homolog HPC8 Secreted cement gland protein XAG-2 homolog |
| Molecular Weight(MW) | 19979 Da |
| Function | Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth |
| Tissue Specificity | Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines |
| Cellular Localization | Secreted. Endoplasmic reticulum (By similarity) |
| Entrez Gene | 10551 |
|---|
Application
Western blot analysis of AGR2 Antibody (N-term) in T47D cell line lysates (35ug/lane). AGR2 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of AGR2(arrow) using rabbit polyclonal AGR2 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AGR2 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Anterior Gradient 2 (AGR2) Antibody (N-term)(Cat#AP6279a) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
