Application
AP1M1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the AP1M1 antibody detected the AP1M1 protein (arrow).Application
AP1M1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of AP1M1 Antibody (Center)(Cat#AP12036c) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | AP1M1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP1M1; CLTNM; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; Adaptor protein complex AP-1 mu-1 subunit; Adaptor-related protein complex 1 mu-1 subunit; Clathrin assembly protein complex 1 medium chain 1; Clathrin coat assembly protein AP47; Cla |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLTNM |
|---|---|
| Alternative Names | AP1M1 CLTNM AP-1 complex subunit mu-1 AP-mu chain family member mu1A Adaptor protein complex AP-1 mu-1 subunit Adaptor-related protein complex 1 mu-1 subunit Clathrin assembly protein complex 1 medium chain 1 Clathrin coat assembly protein AP47 Cla |
| Molecular Weight(MW) | 48587 Da |
| Function | Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules |
| Tissue Specificity | This AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-235 amino acids from the Central region of human AP1M1. |
| Cellular Localization | Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex |
| Entrez Gene | 8907 |
|---|---|
| Nucleotide Accession | NP_115882.1 |
Application
AP1M1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the AP1M1 antibody detected the AP1M1 protein (arrow).
Application
AP1M1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of AP1M1 Antibody (Center)(Cat#AP12036c) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
