Application
Western blot analysis of AP1S1 Antibody (N-Term) in MDA-MB231 cell line lysates (35ug/lane).AP1S1 (arrow) was detected using the purified Pab.Application
AP1S1 Antibody (N-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP1S1 Antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
AP1S1 Antibody (N-Term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AP1S1 Antibody (N-Term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP1S1; AP19; CLAPS1; AP-1 complex subunit sigma-1A; Adapter-related protein complex 1 sigma-1A subunit; Adaptor protein complex AP-1 sigma-1A subunit; Clathrin assembly protein complex 1 sigma-1A small chain; Clathrin coat assembly protein AP19; Golgi adap |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AP19 CLAPS1 |
|---|---|
| Alternative Names | AP1S1 AP19 CLAPS1 AP-1 complex subunit sigma-1A Adapter-related protein complex 1 sigma-1A subunit Adaptor protein complex AP-1 sigma-1A subunit Clathrin assembly protein complex 1 sigma-1A small chain Clathrin coat assembly protein AP19 Golgi adap |
| Molecular Weight(MW) | 18733 Da |
| Function | Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex |
| Entrez Gene | 1174 |
|---|
Application
Western blot analysis of AP1S1 Antibody (N-Term) in MDA-MB231 cell line lysates (35ug/lane).AP1S1 (arrow) was detected using the purified Pab.
Application
AP1S1 Antibody (N-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP1S1 Antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
AP1S1 Antibody (N-Term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
