Application
A. Western blot analysis on (A) MCF-7, (B) SKBR-3 cell lysates using anti-AP2 mu1 RabMAb (Cat. #AJ1046a), dilution 1:1000.| Product Name | AP2 mu1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP2M1, CLAPM1, KIAA0109, AP-2 complex subunit mu, Adapter-related protein complex 2 mu subunit;Adaptor protein complex AP-2 subunit mu;Adaptin-mu2;AP-2 mu chain;Plasma membrane adaptor AP-2 50 kDa protein;HA2 50 kDa subunit;Clathrin assembly protein co |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2695Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000~50000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLAPM1 KIAA0109 |
|---|---|
| Alternative Names | AP2M1 CLAPM1 KIAA0109 AP-2 complex subunit mu Adapter-related protein complex 2 mu subunit Adaptor protein complex AP-2 subunit mu Adaptin-mu2 AP-2 mu chain Plasma membrane adaptor AP-2 50 kDa protein HA2 50 kDa subunit Clathrin assembly protein co |
| Molecular Weight(MW) | 49655 Da |
| Function | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X- X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane- associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-terminus of human AP2 mu1 was used as an immunogen. |
| Cellular Localization | Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV (By similarity) |
| Entrez Gene | 1173 |
|---|
Application
A. Western blot analysis on (A) MCF-7, (B) SKBR-3 cell lysates using anti-AP2 mu1 RabMAb (Cat. #AJ1046a), dilution 1:1000.| Application Notes | WB:1:5000~50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
