Application
AP3S1 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).Application
AP3S1 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).Application
AP3S1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP3S1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | AP3S1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP3S1; CLAPS3; AP-3 complex subunit sigma-1; AP-3 complex subunit sigma-3A; Adapter-related protein complex 3 sigma-1 subunit; Clathrin-associated/assembly/adapter protein, small 3; Sigma-3A-adaptin; Sigma-adaptin 3a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLAPS3 |
|---|---|
| Alternative Names | AP3S1 CLAPS3 AP-3 complex subunit sigma-1 AP-3 complex subunit sigma-3A Adapter-related protein complex 3 sigma-1 subunit Clathrin-associated/assembly/adapter protein small 3 Sigma-3A-adaptin Sigma-adaptin 3a |
| Molecular Weight(MW) | 21732 Da |
| Function | Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals |
| Tissue Specificity | Present in all adult tissues examined. |
| Cellular Localization | Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex |
| Entrez Gene | 1176 |
|---|---|
| Nucleotide Accession | NP_001275.1 |
Application
AP3S1 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).
Application
AP3S1 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).
Application
AP3S1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP3S1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
