Application
A. Western blot analysis on HeLa cell lysate using anti-Apaf-1 RabMAb (Cat. #AJ1048a); dilution 1:500.Application
B. Immunohistochemical analysis of paraffin-embedded stomach adenocarcinoma using anti-Apaf-1 RabMAb (Cat. #AJ1048a).| Product Name | Apaf-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APAF1, KIAA0413, Apoptotic protease-activating factor 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E38 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| IHC:1:50~1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0413 |
|---|---|
| Alternative Names | APAF1 KIAA0413 Apoptotic protease-activating factor 1 |
| Molecular Weight(MW) | 141840 Da |
| Function | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis |
| Tissue Specificity | Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 317 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Apaf-1 RabMAb (Cat. #AJ1048a); dilution 1:500.
Application
B. Immunohistochemical analysis of paraffin-embedded stomach adenocarcinoma using anti-Apaf-1 RabMAb (Cat. #AJ1048a).| Application Notes | IHC:1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
