Application
Western blot analysis of APBB1IP Antibody (Center) in 293 cell line lysates (35ug/lane). APBB1IP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with APBB1IP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
APBB1IP Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | APBB1IP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APBB1IP; PREL1; RARP1; RIAM; Amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; Proline-rich EVH1 ligand 1; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; Retinoic acid-responsive |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PREL1 RARP1 RIAM |
|---|---|
| Alternative Names | APBB1IP PREL1 RARP1 RIAM Amyloid beta A4 precursor protein-binding family B member 1-interacting protein APBB1-interacting protein 1 Proline-rich EVH1 ligand 1 Proline-rich protein 73 Rap1-GTP-interacting adapter molecule Retinoic acid-responsive |
| Molecular Weight(MW) | 73183 Da |
| Function | Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin- induced promoter activities through AP1 and SRE. Mediates Rap1- induced adhesion |
| Tissue Specificity | Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes |
| Cellular Localization | Cell membrane; Peripheral membrane protein (By similarity). Cell projection, lamellipodium (By similarity) Cell junction, focal adhesion (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F-actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases (By similarity) |
| Entrez Gene | 54518 |
|---|
Application
Western blot analysis of APBB1IP Antibody (Center) in 293 cell line lysates (35ug/lane). APBB1IP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with APBB1IP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
APBB1IP Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
