Application
A. Western blot analysis on 293 cell lysate using anti-APC RabMAb (Cat. #AJ1049a), dilution 1:1,000.Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-APC RabMAb (Cat. #AJ1049a).| Product Name | APC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC, DP2.5, Adenomatous polyposis coli protein, Deleted in polyposis 2.5 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP701Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DP2.5 |
|---|---|
| Alternative Names | APC DP2.5 Adenomatous polyposis coli protein Deleted in polyposis 2.5 |
| Molecular Weight(MW) | 311646 Da |
| Function | Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization |
| Tissue Specificity | Expressed in a variety of tissues. |
| Cellular Localization | Cell junction, adherens junction. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle membrane. Cytoplasm. Cell membrane. Note=Associated with the microtubule network at the growing distal tip of microtubules Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane |
| Entrez Gene | 324 |
|---|
Application
A. Western blot analysis on 293 cell lysate using anti-APC RabMAb (Cat. #AJ1049a), dilution 1:1,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-APC RabMAb (Cat. #AJ1049a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
