Application
Western blot analysis of anti-APH1 Pab in mouse brain (left) and HL60 (right) cell line lysate (35ug/lane). APH1(arrow) was detected using the purified Pab (1:50 dilution).| Product Name | APH1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APH1A; PSF; Gamma-secretase subunit APH-1A; Aph-1alpha; Presenilin-stabilization factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PSF |
|---|---|
| Alternative Names | APH1A PSF Gamma-secretase subunit APH-1A Aph-1alpha Presenilin-stabilization factor |
| Molecular Weight(MW) | 28996 Da |
| Function | Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex |
| Tissue Specificity | Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi- pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi |
Application
Western blot analysis of anti-APH1 Pab in mouse brain (left) and HL60 (right) cell line lysate (35ug/lane). APH1(arrow) was detected using the purified Pab (1:50 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
