WB
Western blot analysis of SFPQ using anti-SFPQ antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SFPQ antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for SFPQ at approximately 100 kDa. The expected band size for SFPQ is at 76 kDa.IHC
IHC analysis of SFPQ using anti-SFPQ antibody . SFPQ was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-SFPQ Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF
IF analysis of SFPQ using anti-SFPQ antibody. SFPQ was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SFPQ Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody.ICC/IF
ICC/IF analysis of SFPQ using anti-SFPQ antibody and anti-Beta Tubulin antibody . SFPQ was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SFPQ Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green) were used as secondary antibody.FC
Flow Cytometry analysis of 293T cells using anti-SFPQ antibody . Overlay histogram showing 293T cells stained with A02243-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFPQ Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-SFPQ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SFPQ, identical to the related mouse sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIFIHCWB |
| WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 IHC:1:50-400 IF:1:50-400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SFPQ |
|---|---|
| Gene Synonyms | PSF POMP100 PPP1R140 |
| Gene Full Name | splicing factor proline and glutamine rich |
| Gene Summary | Enables DNA binding activity; histone deacetylase binding activity; and protein homodimerization activity. Involved in several processes, including alternative mRNA splicing, via spliceosome; positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; and regulation of transcription by RNA polymerase II. Acts upstream of or within double-strand break repair via homologous recombination. Located in chromatin; nuclear matrix; and paraspeckles. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | 100 kDa DNA pairing protein hPOMp100 POMP100 PSF PTB associated splicing factor SFPQ |
| Molecular Weight(MW) | 76 kDa(Observed band size:100 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of SFPQ using anti-SFPQ antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SFPQ antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for SFPQ at approximately 100 kDa. The expected band size for SFPQ is at 76 kDa.
IHC
IHC analysis of SFPQ using anti-SFPQ antibody . SFPQ was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-SFPQ Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF
IF analysis of SFPQ using anti-SFPQ antibody. SFPQ was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SFPQ Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody.
ICC/IF
ICC/IF analysis of SFPQ using anti-SFPQ antibody and anti-Beta Tubulin antibody . SFPQ was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SFPQ Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green) were used as secondary antibody.
FC
Flow Cytometry analysis of 293T cells using anti-SFPQ antibody . Overlay histogram showing 293T cells stained with A02243-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFPQ Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 IHC:1:50-400 IF:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
