WB
Western blot analysis of PSF expression in untreated HeLa (A), chemically-treated HeLa (B), K-562 (C) and HCT-116 (D) whole cell lysates. Detection reagent used: m-IgG2a BP-HRP: sc-542731. b-Actin (C4): sc-47778 used as loading control.IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid nodular goiter tissue showing nuclear localizationICC/IF
Immunofluorescence staining of paraformaldehyde-fixed HeLa cells showing nuclear localization| Product Name | Mouse Monoclonal antibody to PSF |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:50-1:500 IHC:1:50-1:500 WB:1:100-1:1000 |
|
| Species Reactivity | Human |
| Purification | Affinity purified |
| Gene Symbol | SFPQ |
|---|---|
| Gene Synonyms | PSF POMP100 PPP1R140 |
| Gene Full Name | splicing factor proline and glutamine rich |
| Gene Summary | Enables DNA binding activity; histone deacetylase binding activity; and protein homodimerization activity. Involved in several processes, including alternative mRNA splicing, via spliceosome; positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; and regulation of transcription by RNA polymerase II. Acts upstream of or within double-strand break repair via homologous recombination. Located in chromatin; nuclear matrix; and paraspeckles. [provided by Alliance of Genome Resources, Jul 2025] |
WB
Western blot analysis of PSF expression in untreated HeLa (A), chemically-treated HeLa (B), K-562 (C) and HCT-116 (D) whole cell lysates. Detection reagent used: m-IgG2a BP-HRP: sc-542731. b-Actin (C4): sc-47778 used as loading control.
IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid nodular goiter tissue showing nuclear localization
ICC/IF
Immunofluorescence staining of paraformaldehyde-fixed HeLa cells showing nuclear localization| Application Notes | ICC/IF:1:50-1:500 IHC:1:50-1:500 WB:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 50 µg IgG2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
