WB
Various lysates were subjected to SDS PAGE followed by western blot with SFPQ antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using SFPQ antibody at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SFPQ antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SFPQ and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Product Name | SFPQ Mouse Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SFPQ protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:2000-1:8000 ICC:1:400-1:1600 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SFPQ |
|---|---|
| Gene Synonyms | PSF POMP100 PPP1R140 |
| Gene Full Name | splicing factor proline and glutamine rich |
| Gene Summary | Enables DNA binding activity; histone deacetylase binding activity; and protein homodimerization activity. Involved in several processes, including alternative mRNA splicing, via spliceosome; positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; and regulation of transcription by RNA polymerase II. Acts upstream of or within double-strand break repair via homologous recombination. Located in chromatin; nuclear matrix; and paraspeckles. [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | 100 kDa DNA-pairing protein 1G4A5 DNA-binding p52/p100 complex 100 kDa subunit Polypyrimidine tract-binding protein-associated-splicing factor POMP 100 |
| Molecular Weight(MW) | 76kDa(Observed Molecular Weight 90-100 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SFPQ antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using SFPQ antibody at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SFPQ antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SFPQ and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Application Notes | WB:1:5000-1:50000 IHC:1:2000-1:8000 ICC:1:400-1:1600 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
