Application
API5 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the API5 antibody detected the API5 protein (arrow).| Product Name | API5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | API5; Apoptosis inhibitor 5; Antiapoptosis clone 11 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; Protein XAGL |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | API5 Apoptosis inhibitor 5 Antiapoptosis clone 11 protein Cell migration-inducing gene 8 protein Fibroblast growth factor 2-interacting factor Protein XAGL |
|---|---|
| Molecular Weight(MW) | 59005 Da |
| Function | Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs |
| Tissue Specificity | Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer |
| Cellular Localization | Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic |
| Entrez Gene | 8539 |
|---|---|
| Nucleotide Accession | NP_006586.1 |
Application
API5 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the API5 antibody detected the API5 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
